Isolation and characterization of α-l-rhamnosidase producing bacterium, Agrococcus sp. bkd37, from a warehouse soil and partial optimization of its culture conditions Pegu Bhaba Kumar, Kardong Devid*, Chetia Pankaj, Chutia Jitu, Gogoi Dip Kumar1 Department of Life Sciences, Dibrugarh University, Dibrugarh - 786 004, Assam (India) 1Biotechnology Division, Central Muga Eri Research & Training Institute, Central Silk Board, Lahdoigarh, Jorhat - 785 700, Assam (India) *e-mail: kardongdevid@dibru.ac.in
Abstract The present study was attempted to identify and partially optimize the culture conditions of a potential α-L-rhamnosidase producing bacterium isolated from warehouse soil of Dibrugarh, Assam (India). Twenty eight bacterial strains were isolated and the isolate BKD37 showed maximum rhamnosidase enzyme activity (10.01 ± 0.001 U mL-1) in crude culture medium. The 16S rRNA gene sequencing and phylogenetic analysis of BKD37 strain showed maximum similarity with that of Agrococcus sp. The culture conditions of strain BKD37 for the production of extracellular rhamnosidase enzyme was partially optimized in a basal medium in shake flask. The maximum rhamnosidase production was achieved after 60 h incubation at 30°C and pH 6.0. The rhamnose and NH4NO3 were found to be the best carbon and nitrogen sources, respectively, in basal medium for α-L rhamnosidase enzyme production. Top Keywords Agrococcus sp, α-L-rhamnosidase, Bacterium, Characterization, Warehouse soil. Top |