In vivo Comparison of Antimicrobial Effectiveness of Octenidine Dihydrochloride and Artemisia annua Plant Extract as Root Canal Irrigant Dr. Sroa Renu Bala1,*, Dr. Devi Pushpa2, Dr. Aggarwal Sangeeta3, Dr. Kumar Baljeet4, Dr. Tandon Namita5 1Professor and Head of Department of Conservative Dentistry and Endodontics, Government Dental College, Amritsar 2Professor and Head of Depatment of Microbiology, Government Medical College, Amritsar 3Medical Officer of Department of Conservative Dentistry and Endodontics, Government Dental College, Amritsar 4Assistant Professor of Department of Conservative Dentistry and Endodontics, Government Dental College, Amritsar 5Post Graduate Student of Department of Conservative Dentistry and Endodontics, Government Dental College, Amritsar *Corresponding author: Dr Renu Bala Sroa, Professor and Head of Department of Conservative Dentistry and Endodontics, Government Dental College, Amritsar
Online published on 16 July, 2018. Abstract Introduction The present in-vivo study was undertaken to evaluate and compare the antibacterial efficacy of Octenidine dihydrochloride and Artemisia annua plant extract as root canal irrigants. Material and Method Forty two non-vital maxillary central incisors were selected from healthy patients and divided into four groups i.e. Group 1 (Saline + Octenidine dihydrochloride) Group 2 (Octenidine dihydrochloride alone) Group 3 (Saline+Artemisia annua plant extract) and group 4 (Artemisia annua plant extract alone). After the root canal access opening first microbiological sample (S1) was collected by introducing two sterile paper points. Samples were placed in thioglycollate broth and peptone water for anaerobic and aerobic culture respectively. The samples were immediately transferred to the laboratory in respective broths for culturing. The canal preparation was carried out following step back technique and sample (S2) was taken after canal preparation was completed. Then final sample (S3) was collected after irrigation with respective irrigant used in each group. The number of colony forming units was counted in each group using digital colony counter. The statistical analysis was carried out using paired T test. Results The reduction in CFU count for aerobic and anaerobic bacteria after final irrigation was maximum in Group1 followed by Group 4, Group 3 and Group2. Statistically highly significant reduction in CFU/mL count was found in Group 1. Conclusion Octenidine dihyrochloride in combination with normal saline had significantly greater antimicrobial effect than Artemisia annua plant extract. Top Keywords Irrigants, Artemisia annua plant extract, Octenidine Dihydrochloride, antimicrobial activity. Top |