Sequencing Analysis of Interleukin-4 gene 590C>T in Patients with Type-2 Diabetes Mellitus Ahmed Intisar Hussein1, Ghali Zafir Hassan1 1Department of Biology, College of education for pure Science, University of Wasit, Iraq Online published on 27 November, 2019. Abstract Background The regulation of immune system at multiple levels is related with IL-4. Diabetes is a global health problem and is the sixth leading cause of death in the world. Aim In this study, we analyze the mutational changes related with polymorphisms of IL-4-590 C>T gene among64 patients with type-2 diabetes melliyus (T2DM) and 26 healthy individuals as control group. Patients and controls were from Wasit province in Iraq. Study design The study design was case-control study. Materials and Method The allele-specific PCR (ARMS-PCR) technique was used to detect the IL-4-590 C>T gene polymorphism (rs2243250). PCR products for IL-4-590 C>T and primers were prepared for sequencing analysis in order to detect mutations and alterations in IL-4-590 C>T gene of patients with T2DM. Results After alignment of product amplification of IL-4 590C>T CC or CT genotypes, there are five transversion substitution mutations in patients with diabetes mellitus table 2. Transversion substitution mutations: thymine to adenine T>A in site 864, Thymine to guanine T>G in site 814, transversion thymine to guanine in site 859 and in site 860. Conclusions We conclude that different transversion mutations at IL-4 gene 590C>T are associated with type-2diabetes mellitus. Top Keywords Interluekin-4, diabetes melltus, polymerase chain reaction, transition, mutation. Top |