Detection of Candida Species Phenotype and Genotype in Diabetes Mellitus Patient in Baquba Teaching Hospital Athab Ahmed M.1, Rasheed Maha Mohammed2, Ahmed luma T.2 1Deparment of Internal Medicine, Collage of Medicine University of Diyala, Iraq 2Department of Microbiology, Collage of Medicine University of Diyala, Iraq Online published on 27 November, 2019. Abstract Background and Purpose Diabetic patients are more susceptible to oral candidiasis infection than non-diabetics due to the factors promoting oral carriage of Candida. Aimed of this study was Detection, isolation and identification of Candida spp. isolated from patients that who have diabetes mellitus. In Diyala province by routine laboratory procedures and molecular techniques based on PCR, Identification genotypes distributions of Candida albicans. Materials and Method Swabs were taken from the mouth of 100 patients and were cultured on Sabouraud dextrose agar (SDA) medium. Than we made sub culture for pure colony, and then we used CHROM agar to differential Candida spp. by change of colures, also we used germ tube for identification of Candida albican. and we used polymerase chain reaction (PCR). The study was performed during first of December 2017 to the end of February 2018. (n=100) of diabetic patients There were taken from Baquba Teaching Hospital, in Baquba city, center of Diyala province. Results The results of PCR shown that the detect of 25S rDNA were 25(83.3%) isolates belonged to the genotype A and 5 (16.6%) genotype C of the C. albicans. There were no results for genotype B. Conclusion C. albicans genotype A is the most frequent genotype in patients followed by genotype C. Top Keywords Diabetes mellitus, Oral candidiasis, CHROMagar Candida, PCR. Top |