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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 618) Last page : ( 624)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00360.8

Antimicrobial Activity of Aqueous, Ethanolic Extracts of Nigella Sativa (Black Cumin) Against Pathogenic Bacteria Isolation from Digestive Tract in Poultry

Majeed Hala Mohammed

Branch of Microbiology, Veterinary Medicine, University of Tikrit, Iraq

Online published on 27 November, 2019.

Abstract

Background

The aim of present study to determine the main causative agent pathogen which cause digestive diseases in poultry and sensitivity of these isolated for antibiotics.

Material and Method

A total 100 Fecal swabs were collected from five felid in Tikrit city during September and October 2017in Microbiology department-College of Veterinary Medicine-Tikrit University. included in this study and out of these.

Results

The results showed to the type and ratio of bacterial isolated were: Ecoil (14.1%), Staphylococcus aureus(12.9%), Pseudomonase aerginosa(6.1%), staphylococcus epidermidis (16.6%), proteus mirabilis (25.8%), enterobacter aerogenes(3.91%), enterococcus fecalis (1.2%), Klebsilla puenmonia (9.2%), salmonella enteritidis and Serratia marcesense (3.7%). The antibiotic sensitivity results showed the Co-trimoxazole and cephalexin are the drugs of choose use for treatment of digestive infection in poultry. The results ethanolic extracts showed ability of Nigella sativa to inhibition growth pathogenic bacteria. The maximum inhibition zone reached 38 mm to ward Enterococcus fecalis at concentration100%.and the range inhibition zone(28, 25)mm at concentration 50%and25%.and minimum inhibition zone22mm toward Klebsiella pneumonia at concentration100%. while the range inhibition zone (21, 18)mm at concentration (50%, 25%) respectively, while the results aqueous extract the maximum inhibition zone reached 32 mm to ward Enterococcus fecalis at concentration100%.while the range inhibition zone(25, 22) mm at concentration 50%and25%.and minimum inhibition zone zero mm were Staphylococcus epidermedis and Pseudomonas aeruginosa at concentration (100%, 50%, 25%) respectively.

Conclusion

Based on the results obtained in this study, it may be concluded that plant extracts of N. sativa have a stronger and broader spectrum of antimicrobial activity against a number of food borne bacteria and the extracts may be used to discover bioactive natural products that may serve as basic source for the development of new antimicrobial compounds to overcome the problem of increasing resistance to known traditional antibiotics.

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Keywords

Antimicrobial, aqueous, ethanolic extracts, black cumin, bacteria, poultry.

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