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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 810) Last page : ( 815)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00394.3

Isolation and Identification of Bacteria from Patients with Eye Infection and Study of Some Inflammatory Cytokines in Patients

Nabat Zainab Nasser1, Al-Kazazz Zainab Kareem1, Mokif Thanaa Abdulmahdi1

1Al-Furat Al Awsat Technical University, Babylon Technical Institute, Iraq

Online published on 27 November, 2019.

Abstract

Background

The eye is one of the important sensation members in our life. Health guidelines that are given to eye soundness and neatness are important because of many factors where dust, high temperature, microorganisms and other agents can cause many diseases of the eye which may lead to blindness. This study included the investigation of the bacterial species causing inflammation of the eye which include conjunctivitis, eye lid infection and cornea at different age groups.

Method

One hundred and forty three swabs were collected from eyes of infected people in addition to 110 blood samples of people who visited Al-Hilla teaching hospital and Alnoor hospital for Children from December 2017 to Jun 2018. Culture investigations showed 137 positive bacterial growth and 6 without growth. Staphylococcus aureus was the most common isolate 48(35.0%) of the total samples followed by E.coli 20(14.59%) followed by S. pneumoniae 18(13.13%) followed by S. epidermis 17(12.4%) followed by Enterococcus 10(7.299%) followed by K. pneumoniae 8(5.8 3%) followed by H. influenzae 7(5.1%) followed by P. aeruginosae 6(4.37%) followed by N. gonorrhoeae 2(1.45%). Also, the study transacted with determination of cytokines concentration.

Results and Conclusion

IL-8 showed elevated concentrations particularly in age group (1–10) year as it reached 282.67±20.53pg/ml compared to controls 134±97.51pg/ml. Also, IL-1 concentration was higher in patients than in controls particularly in age group (51–60) years as it reached 352.67±46.49pg/ml compared to controls 270±9.54.

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Keywords

Eye infection, IL-1, 1L-8, conjunctivitis, bacterial infection.

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