Association of IL-6 gene−174 G/C (rs1800795) Polymorphism with Juvenile Idiopathic Arthritis in Iraqi Patients Al-Harbi Sawsan Jassim1, Hadi Maysaa Adil2,*, Rashid Kareem Hameed2 1Department of Anatomy, College of Medicine, University of Babylon, Iraq 2Department of Biology, College of Science, University of Babylon, Iraq *Corresponding author, Maysaa Adil Hadi, email: mysadil2015@gmail.com
Online published on 27 November, 2019. Abstract Background Interleukin-6 is the major pro-inflammatory cytokine which exerts multiple effects within the disease activity in juvenile idiopathic arthritis and rheumatoid arthritis patients. The aim of this study was to assess the association between IL-6 gene-174 G/C promoter polymorphism with juvenile idiopathic arthritis and rheumatoid arthritis diseases in Iraqi patients. Method A case-control study was carried out on 82 patients with juvenile idiopathic arthritis and rheumatoid arthritis disease at Mirjan Teaching Hospital in Babylon Province, Iraq. All patients and age matched apparently healthy controls had the same ethnic group (Arabic). The genotyping of IL-6 gene-174 G/C promoter polymorphism with allele frequency between the two-group patient and control were detected using PCR-RFLP technique. Results A Significant increase (P<0.001) was observed in GC genotype in both juvenile idiopathic arthritis and rheumatoid arthritis patients when compared with their respective controls. The frequencies of GG, GC and CC genotypes in juvenile idiopathic arthritis patients were 22.9%, 62.9% and 14.2%, respectively, while in rheumatoid arthritis patients were 25.5%, 61.7% and 12.8%, respectively. Conclusion A significant association of −174 G/C (rs1800795) IL-6 gene polymorphism with juvenile idiopathic arthritis and rheumatoid arthritis in Iraqi population in comparison with control groups was found with higher frequencies of GC genotype and may be as a risk factor in both juvenile idiopathic arthritis and rheumatoid arthritis Iraqi patients compared to controls. Top Keywords Juvenile idiopathic arthritis, rheumatoid arthritis, −174G/C (rs1800795) IL-6 gene polymorphism, PCR-RFLP technique. Top |