The Sequence of TP53 Gene in Anemic Patients Associated with Toxoplasmosis Hassan Salah Mahdi* Department of Medical Laboratory Techniques, College of Health and Medical Technology, Middle Technical University, Iraq *Corresponding author: Salah Mahdi Hassan, Email: Salahmad00ss00@gmail.com
Online published on 27 November, 2019. Abstract Background Toxoplasma gondii is a risky parasite to humans since it causes severe damage to the tissues and organs in the body. This parasite therefore usually enters a dormant state to form small cysts in different tissues of human's body. The parasite Toxoplasma gondii has the ability to modulate microenvironments of infected hosts and it can invade vital body organs like the central nervous system (CNS). The aim of current study was to do screening and IgM tests in the sera of anemic patients who were suspected to be infected with toxoplasmosis. Method A total of eighty whole blood samples were collected from patients complaining from anemia. Eighteen out of 80 patients were found to have toxoplasmosis. Results Distribution of anemic patients according to demographical data showed that the highest number of anemic patients was within the age group (15–45) years, while the distribution of anemic patients according the gender showed that 36(45.0%) were males and 44(55.0%) were females. Also, the distribution of anemic patients according to residency demonstrated that 56(70.0%) were rural and 24(30.0%) were urban. Study of relationship between Anti-Toxoplasma antibodies IgM and hematological parameters showed a highly significant difference in MCH (25.82±6.18), MCV (81.12±40.47) and HGB (12.32±9.09), while MCHC was (273.34±85.19), P<0.01 (HS). In 18 anemic and toxoplasmosis patients, mutation occurred in anemic patients in gene P53 at codons 157, 248 and 273 positions. The nucleotide GAG was changed to GT. Conclusion Toxoplasmosis was found to be the cause of the genetic change that occurred to the gene P53 and there was a reading of the codon encoding for the production of abnormal blood cells. Top Keywords Anemic patients, mutation, toxoplasmosis, IgM, hematological parameters. Top |