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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 1151) Last page : ( 1155)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00456.0

Detection Some Pathogenic Bacteria Causing UTI in Type 2 Diabetic Patients and Determine Some Resistance Genes in Escherichia coli by PCR in Kirkuk City

Afram Asma Samea1,*, Abdulsahib Wafaa2

1Department of Biology, College of Science, Kirkuk University, Iraq

2Microbiologists, Iraqi Ministry of Health, Iraq

*Corresponding author: Asma Samea Afram. Department of Biology, College of Science, Kirkuk University, Iraq

Online published on 27 November, 2019.

Abstract

Determine some types of bacteria that cause urinary tract infection among type 2 diabetes mellitus patients. A cross-sectional study was carried out among 210 patients selected by using non probability sampling (purposive sampling). According to study findings females were 64.3% of the study sample, most of them were an elderly age, 26.2% of them had positive urine culture and Escherichia coli was the most common cause of UTI and then Klebsiella pneumoniae and Staphylococcus aureus. Sensitivity tests were done to determine resistance of bacteria to antibiotic that used in this study and determine some of the resistance genes in E. coli. The gyrA gene and dfrA1 gene are found in resistance isolates of E. coli to Nalidixic acid and Trimethoprim respectively.

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Keywords

Urinary Tract Infection, Type 2 Diabetes Mellitus, Resistance genes, PCR.

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