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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 1252) Last page : ( 1256)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00473.0

Effect of Aqueous Extract of Clove Syzygiumaromaticum Against Pathogenic Fungus Alternariaspp. Isolated From Skin Infections

Obaidal-Hachami Asia Naji1, Hassoni Jaafar Jumaah2

1Biology Department, College of Education for Pure Science, University of Wasit

2College of Dentistry, University of Wasit, Iraq

Online published on 27 November, 2019.

Abstract

The Aim of this Study are to evaluatethe potential antifungal activity of clove aqueous extracts against Alternaria spp. as well as, the smallest concentration capable of inhibiting or preventing their growth, during the period from April 2017 to April 2018, cross sectional study of 48 patients done with 26 of them established diagnosis with A.spp, from them5 females 19.3% and 21 males 80.7% with ages range of 17–55 years, study was done in Al-Karama Teaching Hospital, Altenariaspecies isolated from skin lesion taken from patient in dermatology department in the hospital, the lesion was excised and cultured on potato dextrose agars(PDA), and sabaraud dextrose agar (SDA) colonies developed, effect of clove aqueous extract with three concentrations (5%, 10% and 20%) was assessed by agar diffusion plate method, The radial growth of colonies has been measured and on the basis of these values the percentage of inhibition of colony growth has been calculated, the outcome displayed as tables and figures, the antifungal activities of aqueous extract of clove against Alternaria spp. Was assessed by measuring diameter of colonies in PDA and, SDA which found to be 4cm in PDA with 44.4% growth inhibition percentage, and colony diameter was 5cm in SDA at 5% concentration with 55.5% growth inhibition percentage, comparing with control which show 9 cm colony diameter in both PDA, and SDA, While no growth detected in both SDA and PDA at 10% and 20% concentration comparing with control with 100% growth inhibition percentage Our result shows growth dependent effect on extract concentrations, with decrease growth by increase concentration of extract in conclusions the findings of the present study indicate that the clove aqueous extract in different concentration has interesting potential effect as a natural therapeutic option against fungi that are pathogenic to humans

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Keywords

Clove extract, Alternariaspp, Syzygiumaromaticum, Antifungal, Eugenol.

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