Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 1516) Last page : ( 1520)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00517.6

Direct Molecular Detection of Gardnerella Vaginalis and Lactobacillus Spp. in Women with Bacteria Vaginosis

Al-Bayati Asmaa M. S.

Ph. D. Medical Microbiology, Department of Medical Lab, Technical College, Northern Technical University, Iraq

Online published on 27 November, 2019.

Abstract

Bacterial vaginosis (BV) is considered to be the most frequent vaginal infectious disorder in women of childbearing age. The aim of this study was direct detect of Gardendale vaginalis and Lactobacilli in vaginal mucus of suspected women. A total of 60 baseline vaginal swabs were obtained from 20 subjects with BV and 40 subjects without BV seen between March 2018 and November 2019. In this prospective study, participants attending private clinics in Kirkuk city-North of Iraq BV was defined using Amsel's clinical criteria for all subjects (with the presence of at least three of four criteria needed to establish a diagnosis of BV). In addition, Nugent criteria (Gram stain score ≥ 7) were done to evaluate BV status in all subjects attending the clinics. Vaginal swab samples were collected from each women in the study and stored for molecular detection of Gardendale vaginalis and Lactobacilli by multiplex real time PCR. The presented study found that 80% of BV patients were suffered from previous abortion and 20% haven't this history while 7.5% of women without BV infection have history of abortion. In this study, 50% of women infected with BV were within the age group 30–39 year followed by the age group 20–29 years (35%) and 45% of women without BV infection were within the age group 30–39 year. In the present study, women with clinically diagnosed with BV were 90% positive by multiples RT-PCR, 2% were with mixed (Gardendale vaginalis and Lactobacillus) and no pure infection with Lactobacilli was occurred BV positive women, while the study showed that 92.5% of BV negative women were Lactobacilli positive by multiples RT-PCR, 5% of them showed mixed positive by multiples RT-PCR and 2.5% were with Gardendale vaginalis. The result was highly significant.

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Keywords

Prostate cancer, BK polyomavirus, BKPyV.

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