Extrinsic Factors Governing The Spore Encumberance of Indigenous Population of Pasteuria Hyper-Parasitizing The Root-Knot Nematode, MeloidogyneSpecies Kamra Anju*, Pawar Swapnil, Mohan Sharad Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India *Corresponding author-Email: anjukamra@yahoo.com
Online published on 29 August, 2017. Abstract Host specificity of an indigenous isolate of P. penetrans infecting M. incognita was tested against four species of Meloidogyne, developmental stages of M. incognita and infective stages of Heterodera cajani, H. avenae and Rotylenchulus reniformis. Species specificity was observed with significantly higher spore attachment recorded on J2s of M. incognita (6.25/J2) followed by M. javanica (3.90/J2), M. hapla (1.10/J2)and M. graminicola (0.90/J2). No cross-generic spore attachment was recorded. Spore encumberance was stage specific as besides J2, none of the developmental stage of M. incognita (J3, J4 and males) exhibited any spore on its cuticle. A pre-exposure of spores to 50°C for 10 minutes resulted in significant decline in spore attachment. Incubation of spores in polyhouse soil revealed a gradual decline in spore attachment on J2 of M. incognita. The preincubation of Pasteuria isolate with soil bacteria viz. Bacillus cereus, B. subtilis and B. pumilus, resulted in a reduction of 97.23, 94.60 and 96.57%, respectively, in spore attachment compared to untreated control. Top Keywords Pasteuria, Meloidogyne incognita, developmental stages, M.javanica, M. hapla, M. graminicola, Heterodera cajani, Rotylenchulus reniformis, Bacillus cereus, B. subtilis, B. pumilus. Top |