Evaluation of Southern Root-Knot Nematode Responsive Promoter Against Other Important Plant Nematodes Singh Ashish Kumar1, Kumar Anil2, Joshi Ila2, Thorat Yogesh E.1, Jain Pradeep Kumar2, Sirohi Anil1,*, Pankaj1 1Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India 2NRCPB, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India *Corresponding author, e-mail: anilsirohi@yahoo.com
Online published on 29 August, 2017. Abstract A Meloidogyne incognita responsive root specific promoter was isolated from Arabidopsis root, was validated against rice root-knot nematode (M. graminicola) and reniform nematode (Rotylenchulus reniformis) at 7, 14, 21, and 28 day post inoculation (dpi) using GUS assay and qRT-PCR against both the nematodes. GUS expression was evident in Arabidopsis root against the infection of both nematode, M. graminicola and R. reniformis at each time interval. These results help us to conclude that this promoter can be utilized in engineering resistance against rice root-knot nematode (M. graminicola) and reniform nematode (R. reniformis) in commercial crops. Top Keywords Plant parasitic nematodes, promoter, GUS, qRT-PCR, Meloidogyne graminicola, Rotylenchulus reniformis, Arabidopsis. Top |