In Vitro and Molecular Assessment for Lead Tolerance in Aloevera L. Callus Al-Roomi Rana A. Hameed1,*, Al-Oubaidi Hashim K. Mohammed1, Hameed Rana Hadi1 1Department of Biology, College of Science, Al-Mustansiriyah University, Iraq *Corresponding Author: Rana A. Hameed Al-Roomi, Department of Biology, College of Science, Al-Mustansiriyah University, Iraq, e-mail: alroomir@yahoo.com, alroomi@uomustansiriyah.edu.iq
Online published on 23 December, 2019. Abstract A biotic ecological stress element has negative influences on various characteristics of plant growth, crop productivity, and development. In the past few decades there were advances in plant molecular biology, physiology, tissue culture, and genetics, have greatly improved our preception of plant response to a biotic stress situations. In vitro culture may produce a suitable method for selection of heavy metaltolerant plantlets. Aloe callus were grown on media supplemented with 0.5, 1.0, 1.5, and 2.0 mg/l lead nitrate. Level of lead bioaccumulation, mineral status, and growth parameters of cultured callus were investigated. Results showed an increase in Pb accumulation with increase of lead concentration treatment, reaching 124.633 ppm. at 1.5 mg/l Pb, in regard to PCR analysis, experiment included catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR) genes. Results revealed that the number of amplicons varied with increase of lead concentration treatment with the compare of control treatment. Top Keywords In vitro, lead tolerance, Aloe, callus. Top |