Finding of Helicobacter Pylori through Biopsy Trials to Gastro intestinal Region Infections in Iraq Patients Ali Mariam Kareem1, Shia Jaafar Sataar2 1Medical Microbiology, Department of Microbiology, College of Medicine, Baghdad University, Iraq 2Pharmacy Ministry of Health, Baghdad, Iraq Online published on 23 December, 2019. Abstract This research work focus on determination of the infection rates of Helicobacter pylori (H.pylori) in Baghdad Iraq. This work was achieved in 2017 from February to December 2017, a 100 samples were involved in this research, the age of patients in this study were ranged from 15 to 65 years. biopsy Gastric tissue were collected from one hundred patients suffering symptoms of severe gastrointestinal tract diseases (GITD), All patients were subject to the tests included in the current study and done gastro-endoscopy in the Gastrointestinal-tract and Liver Disease Hospital, and Al-Yarmouk Hospital, Baghdad, Iraq. Direct examination of H. pylori from biopsy Gastric tissue using the Rapid Urease Test (RUT), samples were cultured and PCR was achieved after that in sequence. The outcome data shows that 42% of the samples in study were respond positively to PCR while 32% of them were positively to RUT and 24% were positively to culture. Molecular analysis was used to detect each sample and it was found that the positive percent in males is much higher than females and the (25–45) year's group has the highest rate in positive cases. According to type of GITD and H. pylori diagnostic gene (ureC), showed that 15(41.7%) 18(46.2%), 8(40%), 1(20%) cases of Gastritis, Gastritis & doudenitis, Gastropath ulcer, oseophagas tumor respectively. Top Keywords Helicobacter pylori, severe gastro-intestinal tract diseases, Biopsy. Top |