Extraction, Purification and Genotyping of β-LactamAse from Local Isolate of Acinetobacter Baumannii Abdul-Hussein Taif Majid1, Almaali Hasan Mahmood Abo2, Al-Jumaily Essam F.1, Saadedin Shurook M. K.1, Al-Wattar Wifaq M.3 1Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Iraq 2College of Pharmacy, University of Karbala, Iraq 3Department of Microbiology, College of Medicine, University of Baghdad, Iraq Online published on 23 December, 2019. Abstract Beta-lactamase was purified from local isolate of Acinetobacter baumannii by several steps included ion-exchange chromatography by using DEAE-Cellulose and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 11.72, 55.12% respectively. The characterization of the purified Beta-lactamase showed that the molecular weight was about 44, 668 KD as determined by gel filtration. The Acinetobacter baumannii gene (blaTEM) responsible for β-lactamases detection was by PCR. They recorded positive results reaching 39(86.66%), from the isolates. Top Keywords β-Lactamase, Acinetobacter baumannii, Molecular weight, genotyping. Top |