Detection of Cytolysis Peptide Toxin Gene (Candidalysin) in Candida albicans Isolates Al-zubaidi Sara K.* Department of Medical Lab. Technology, Al-Zahrawi University College, Karbala, Iraq *Corresponding Author: Sara K. Al-zubaidi, Department of Medical Lab. Technology, Al-Zahrawi University College, Karbala, Iraq, Email: saraalzubaidi89@gmail.com
Online published on 23 December, 2019. Abstract This study was launched to identify the presence of the gene; ece1 (extent of cell elongation 1), encodes for a precursor protein related to the candidalysin, a cytolytic peptide toxin important for the induction of mucosal-related infections, in isolates of Candida albicans. Initially, 30 isolates of C. albicans were provided by Merjan Teaching Hospital, Hilla City, Iraq. These isolates had been originally recovered from 10 children suffered mouth thrush (MT) infection, 10 women with infection of vaginal candidiasis (VC), and 10 patients with immunocompromised-based candida (ICC) infection. The samples were Lab-based processed using a polymerase chain reaction (PCR) method to amplify an ece1-specific-362bp region. The results revealed the presence of the current gene in 4 (40%) of the ICC samples, 1 (10%) of the VC samples, and 3 (30%) of the MT samples. The current findings of the current investigation indicate the presence of the C. albicans candidalysin virulence gene in the tested isolates from Hilla City, Iraq. The work also suggests performing future studies or adopting novel control standards to overcome the virulence processes initiated by this gene. Top Keywords Candida albicans, candidalysin, immunocompromised candidiasis, mouth thrush, vaginal candidiasis. Top |