Molecular Investigation of Aminoglycoside Modifying Enzyme among Aminoglycoside-Resistant Uropathogenic Escherichia Coli Isolates from Najaf Hospitals, Iraq Alm'amoori Khadeeja Obaid1, Hadi Zainab Jaber1, Almohana Ali Muhsin1,* 1Faculty of Medicine, Kufa University, Iraq *Corresponding Author: Ali Muhsin Almohana, Faculty of Medicine, Kufa University, Iraq, Email: alnajafmalak@gmail.com
Online published on 23 December, 2019. Abstract Background E coli is the most common uropathogen for uncomplicated UTIs (75–95%). Plasmid-mediated AMEs were reported to be the widespread among Gram-negative isolates resistant to aminoglycosides. The purpose of this study is to investigate the occurrence of genes encoding AMEs in aminoglycosides resistance UPEC isolates. Method A cross sectional study of the participate patients their approval for direction usage their specimen. The susceptibility test by disk diffusion for UPEC were isolated from urine and thirteen plasmid-mediated AMEs aac(3’)-I, aac(3’)-II, aac(3)-III, aac(3’)-IV, ant(2′′)-I, ant(3″)-1, ant(4′)-IIa, acc(6’)-Ib, acc(6’)-IIb, aph(3’)-I, aph(3’)-IIa, aph(3’)-Ib, and aph(3′)-VI were screened by mono-and tri-plex PCR. Results 298 was significant bacteruria, there were 132 clinical isolates of uropathogenic E. coli, among these 60(45.5%) co resistant to at least two aminoglycoside antibiotics were selected varied from (100%) kanamycin to (53.3% (amikacin. It was found that 98.3%, 95.0%, 90.0%, 86.7%, 51.7%, 36.7%, and 10% of isolates were harbored aac(6’)-Ib, aph(3)-I, aph(3′)-IIa, aph(3′)-Ib, ant(3″)-I, (aac(3)-II, and aac(3)-IV, respectively. Conclusion AME genes were present in all of the UPEC isolates and the first aph(3′)-I, aph(3’)-Ib, aph(3′)-IIa and ant(3″)-I determinants in Iraq. Top Keywords Plasmid-mediated AMEs, uropathogenic E. coli, aac(6’)-Ib, aph(3)-I, aph(3′)-IIa, aph(3′)-Ib, ant(3″)-I, (aac(3)-II, and aac(3)-IV. Top |