Evaluation of CK20 and CD8 Tumor Markers in Patients with Colorectal Cancer by Using Immunohistochemical Technique AL-Rubaiawi Ahlam Shakir Hammood1,*, Haba Mukhtar Khamis1, AL-Hadad Laith Raouf Ahmed2, AL-Khafaji Ali Hussein Mohammed3 1Department of Biology, College of Science for Women, University of Baghdad, Iraq 2Consultant Digestive Surgery, GIT Hospital, FRCS, FACS, Iraq 3M. B. ch. B, Ph D (Path.), College of Dentistry, Private Uruk University, Iraq *Corresponding Author: Ahlam Shakir Hammood AL-Rubaiawi Department of Biology, College of Science for Women, University of Baghdad, Iraq, Email: ahlamshaker2014@gmail.com
Online published on 23 December, 2019. Abstract Colorectal cancer (CRC) is one of the common malignant tumors of gastrointestinal tract (GIT). The incidence rate of this cancer increased in frequency in the last few years. This study was carried out to investigate the possible association of some risk factors with CRC such as (age, gender, smoking, inflammatory bowel disease, and ulcerative colitis). In this study we analyzed using immunohistochemistry technique of the following markers for 30 selected cases: cytokeratin 20 (CK20), and cytotoxic T-lymphocyte (CD8), the results showed that CD8 and CK20 are positive immunostaining markers. CD8 expression: Results indicated that CD8 levels were positive in the all cases studied. In term of scores, CRC patients with the score ++ (moderate) represented the highest percentage 14 (46.67%) compared with patients with score+ (weak) 10(33.33%) and score+++ (strong) 6(20.00), the statistical analysis showed increase significant in percentage of patients with score++ as compared with score+ and score+++ at (P<0.05). CK20 expression: In this study the positive immunohistochemical expression of CK20 was significantly higher in CRC patients with score ++ (moderate) 16 (53.33) in comparison with CRC patients with scores + (weak) 9 (30.00) and score +++ (strong) 5 (16.67) at (P<0.05). Top Keywords Colorectal cancer, Immunohistochemistry, Cytotoxic T-lymphocyte, Cytokeratin 20. Top |