The Cytotoxic Effect of Zinc Oxide on Colon Cancer Cell Lines in Vitro Jasim Shihab Ahmed1, Saleh Nihad A.2,* 1Department of Physics, College of Science, Kufa University, Iraq 2Department of Physics, College of Science, University of Babylon, Iraq *Corresponding Author: Nihad A. Saleh, Department of Physics, College of Science, University of Babylon, Iraq, Email: Nb200320022000@yahoo.com
Online published on 23 December, 2019. Abstract Background The small size and surface properties of Zinc Oxide Nanoparticles enabled them to diffuse easily through, and localize inside, tumor cells. The aim of current study was to evaluate the anticancer effects of Zinc Oxide Nanoparticles on a colonic cancer cell line in vitro with the use of Methyl Thiazolyl Tetrazolium assay. Method The cytotoxic effects of of Zinc Oxide Nanoparticles against colon cancer were planned with a serial doubling dilution concentrations (μg/mL). The microscopic features of apoptotic cell were shown and viability was measured by using Methyl Thiazolyl Tetrazolium assay, a reliable and common colorimetric test for evaluation of cell viability. Results According to cell viability assays, 15μg/mL and 8μg/mL of Zinc Oxide Nanoparticles were found to be highly toxic with the most potent antitumor activity on colonic cancer cell line. In addition, the obtained results showed a major reduction in cell viability and increased apoptosis comparison with normal cell line by same doubled increase concentrations. Conclusion ZnONPs can be used as anti-cancer agents in treatment modalities. They were able to decrease tumor growth in cancer cell lines. Top Keywords MTT Assay, HCT116 colon cancer cell line, Zinc Oxide Nanoparticles, Cytotoxicity, Anticancer. Top |