Combination of Gold Nanoparticles Conjugated Lectin and Using it for Development the Antibacterial Activity of Lectin Purified from Acinetobacter baumannii Muslim Sahira Nsayef1, Ali Alaa Naseer Mohammed1,* 1Department of Biology, College of Science, Mustansiryiah University, Iraq *Corresponding Author: Alaa Naseer Mohammed Ali, Department of Biology, College of Science, Mustansiryiah University, Iraq, Email: alaan29775@gmail.com
Online published on 23 December, 2019. Abstract Lectins are glycoproteins of non-immune source which take a large range of use in medical area. In the present search a new strain, Acinetobacter baumannii c13 took from chicken carcasses samples gave the top production level of lectin by microscopic glass slide and microtiter plate methods. Lectin was purified to similarity by ammonium sulfate at 35% capacity followed by DEAE-cellulose ion exchange chromatography and sephadex G-150 gel filtration chromatography with 45.3 fold of purification and a harvest of 57.1%. Au nanoparticles was made by laser ablation and conjugated to lectin by method of pulses. The Au nanoparticles conjugated lectin led to development of lectin action versus each tested UTI affecting in contrast with ciprofloxacin antibiotic that used for therapy of UTI inflammation and purified lectin, where Au nanoparticles conjugated lectin look great effectiveness versus Enterobacter sp. followed by Salmonella sp. and Streptococcus sp. in contrast with the more tested bacteria. Thus Au nanoparticles conjugated lectin can be used as a suitable antibacterial agent for the action of growing urinary tract infections. Top Keywords Acinetobacter baumannii, lectin, Au nanoparticles. Top |