Sequencing of the Exon 17 C/T, Intron 3 and Intron 8 in INSR (Insulin Receptor Gene) to Identification New SNPs in Iraqi Women with Polycystic Ovarian Syndrome (PCOS) Mohammad Noor H.1,*, AL-Kazaz Abdul Kareem A.1 1Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq *Correspondence author: Noor H. Mohammad E.mail: noorgardinia@yahoo.com
Online published on 8 March, 2019. Abstract In this case-control design study, 46 clinically diagnosed Polycystic Ovarian Syndrome (PCOS) in Iraqi women and 20 healthy Iraqi female were enrolled for analysis of the Exon 17 C/T Single Nucleotide Polymorphisms in Insulin Receptor Gene (INSR) gene as predisposing molecular marker for PCOS and its relationship with diabetes. Genomic DNA was extracted from whole blood of each subject using wizard genomic DNA purification kit. Specific primers for Single Nucleotide Polymorphism (SNPs) analysis of INSR Exon 17, Intron 8 and 3 were used Single Nucleotide Polymorphism-Polymerase Chain Reaction (SNPs-PCR) amplification reaction. Post PCR Restriction Fragment Length Polymorphism (PCR-RFLP) was done for PCR-Products using the Restriction Enzyme PMLI. In conclusion, the Exon 17 C/T Single Nucleotide Polymorphism in INSR gene can consider as predisposing molecular marker for PCOS that can transmitted offspring, colorectal cancer (CRC) and Breast Cancer in Iraqi women and the Intron 8 Single Nucleotide Polymorphism can consider as predisposing molecular marker for PCOS and Diabetes that can be transmitted offspring in Iraqi women. The hormonal values were takes as detected markers for PCOS such as Luteinizing Hormone (LH) values that appears in high level in women with PCOS either they were married or unmarried and the Follicle Stimulating Hormone (FSH) that appear in low levels in women with PCOS. Top Keywords Exon 17 C/T, Intron 3, Intron 8, Insulin Receptor Gene, SNPs, Polycystic Ovarian Syndrome. Top |