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Indian Journal of Public Health Research & Development
Year : 2019, Volume : 10, Issue : 2
First page : ( 553) Last page : ( 558)
Print ISSN : 0976-0245. Online ISSN : 0976-5506.
Article DOI : 10.5958/0976-5506.2019.00350.4

Phenotypic and Molecular Study of mecA Gene in MRSA Isolated from Clinical Cases in Misan Province/Iraq

Aziz Zahid S.1, Hassan Marwa A.2

1Assistant Professor Dr and Head of the Department of Biology

2Postgraduate Student, University of Misan, College of Science, Department of Biology, Misan, Iraq

Online published on 8 March, 2019.

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) is a common strain of S. aureus caused infections lead to increased of risk in clinical outcomes and death, and compared with infections caused by non-resistant strains of the same bacteria patients consume more resources of health-care, where their hospitalization was prolonged because most preferred drugs such as β-lactam antibiotics not have the ability to produce bactericidal effect on this bacteria. Therefore this study aimed to: Isolation and identification of MRSA by using conventional and confirmatory techniques, detection of phenotypic traits related to their antibiotic resistance and estimation the occurrence of mecA gene in identified bacteria. A total of 274 samples, were collected from local hospitals in Misan Province during the period from December 2017 to April 2018. Diagnostic results using microscopic, cultural, biochemical tests and vitek 2 system proved that 106 bacterial isolates belong to Staphylococcus aureus. The susceptibility test of S. aureus isolates was performed toward many types of antibiotics, from all isolates 99(93.4%) were determined as MRSA by oxacillin antibiotic and 93(87.7%) were determined as MRSA by cefoxitin antibiotic. The results of molecular estimation of mecA gene showed that 89(89.9%) and 91(97.8%) from phenotypically positive MRSA isolates had this gene for oxacillin and cefoxitin antibiotics respectively.

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Keywords

Staphylococcus aureus, MRSA, Antibiotic resistance, mecA gene.

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