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Indian Journal of Public Health Research & Development
Year : 2019, Volume : 10, Issue : 2
First page : ( 584) Last page : ( 589)
Print ISSN : 0976-0245. Online ISSN : 0976-5506.
Article DOI : 10.5958/0976-5506.2019.00355.3

Role of microRNA-423 Gene Variation in Women at Higher Risk of Breast Cancer in Tabuk of Saudi Arabia

Mir Rashid1, Balawi Ibrahim Abdullah Al2, Abu-Duhier FM1,*

1Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Prince Fahd Bin Sultan Research Chair, University of Tabuk, Kingdom of Saudi Arabia

2Department of Surgical Oncology, Faculty of Medicine, University of Tabuk

*Corresponding author: Dr FM Abu-Duhier, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Prince Fahd Bin Sultan Research chair, University of Tabuk, Kingdom of Saudi Arabia Email: rashid@ut.edu.sa, rashidmirtabuk@gmai.com

Online published on 8 March, 2019.

Abstract

Aim: Background

MicroRNA has attracted increasing attention due to their possible involvement in the development of various types of cancer. Many studies have been conducted to investigate the association between miR-423 rs6505162C/T and Breast cancer risk; however, the results are not consistent. Therefore, we investigated the association of microRNA-423 rs6505162C>A gene variations with Breast cancer cases, sex match healthy controls and Women at higher risk of breast cancer susceptibility in Saudi women.

Methodology

This study was conducted on 90 subjects among whom 30 were Women at higher risk of breast cancer (WHR), 30 were Breast cancer cases and 30 were gender matched healthy controls. The microRNA-423 rs6505162C>A gene variations was performed using Amplification refractory mutation system PCR method.

Results

A significant difference was observed in genotype distribution between women at higher risk of breast cancer and Breast cancer cases (P=0.04) and between sex match healthy controls and Women at higher risk of breast cancer (P=0.009). The frequencies of all three genotypes CC, CT, TT reported in the women at higher risk of breast cancer were 20%, 43% and 37%, Breast cancer patients 53%, 37% and 10% and in sex matched healthy controls 57%, 30% and 13% respectively. Our results indicated that the miR-423-rs6505162C>T gene variant is associated with an increased risk of Women at higher risk of breast cancer in Codominant inheritance model (OR=0.20, 95% CI=(0.05–0.80) p=0.020, TT vs CC; Dominant model (OR=0.26, 95% CI= (0.86–0.81), p=0.020, CT+TT vs CC) and no significance was reported in Recessive model (OR=0.38, 95%CI= (0.12–1.21), P=0.07, TT vs CC+CT) inheritance models tested. While, the T allele significantly increased the risk in Women at higher risk of breast cancer (OR= 0.38; 95% CI= (0.18–0.78); p=0.009 compared to C allele.

Conclusion

Our findings indicated that microRNA-423 TT genotype and T allele are associated with an increased susceptibility to Breast cancer in high risk women. Furthers studies with larger sample sizes are necessary to confirm our findings.

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Keywords

Women at higher risk of breast cancer, MicroRNA, SNP-single nucleotide polymorphism, .

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