HLA-G and P-Selectin Gene Polymorphism in Women with Recurrent Abortion Having Antiphospholipid Syndrome Hashim Shahad Fadhil1, Ibraheam Israa Adnan1, AL-Bermani Oruba Qutuff1 1Collage of Sciences for women, University of Babylon, Iraq Online published on 8 March, 2019. Abstract Background Antiphospholipid syndrome (APS) is one of the autoimmune disorders in which the vascular thrombosis can cause recurrent pregnancy losses in the first trimester of pregnancy. The aim of this study was to investigate the association of HLA-G 14-bp insertion/deletion and P-selectin N562D gene polymorphism with the thrombosis in pregnant women with APS. Method In this study we recruited 40 pregnant females diagnosed with APS. Blood samples were obtained from all patients and their controls during the period from 1st, September 2017 to 1st, April 2018. DNA was extracted from all samples and HLA-G 14-bp insertion/deletion and P-selectin N562D gene polymorphism were detected using PCR technology. Results HLA-G insertion/deletion gene polymorphism results revealed that the genotype insertion/insertion (II) formed about 17% from patients samples while in control it formed 12%. The genotype insertion/deletion (ID) genotype formed 60% from patients while in the controls it formed 55%. The genotype deletion/deletion (DD) formed 22.5% while in controls it formed 32.5%. In the case of the P-selectin N562D formed DD 42.5%, DN 37.5% and NN 20% in patients while in control it formed 55%, 22.5% and 22.5%, respectively. Conclusion The HLA-G 14-bp insertion/deletion and P-selectin N562D genes polymorphism were not associated with recurrent abortion in Iraqi women with antiphospholipid syndrome. Top Keywords Antiphospholipid syndrome, polymorphism, miscarriage, HLA-G gene, P-selectin, PCR. Top |