Programmed Cell Death Protein-1 (PD-1) Gene Polymorphism and Lung Cancer Ali Mayasa Abdulla1, Hasan Najat Sadeq2, Al-Mayah Qasim S.2 1Medical Laboratory Techniques, Al-Rasheed University College, Iraq 2Medical Research Unit, College of Medicine, Al-Nahrain University, Iraq Online published on 8 March, 2019. Abstract Background Lung cancer is ranked first as a cause of cancer-related mortality worldwide. Programmed cell death protein-1 (PD-1), a type 1 transmembranous receptor It is expressed on activated lymphocytes, natural killer (NK) cells and monocytes. Overexpression of PD-1 gene and high production of the corresponding protein is always parallel with increased PD-1 shedding into soluble form (sPD-1) in the plasma. Measurement of sPD-1 could be useful tool for predicting the activity of PD-1 gene. Method This case-control study included a total of 72 patients with histopathologically confirmed LCa and other 60 family-unrelated and age-matched healthy subjects as controls. DNA was extracted from peripheral blood, and the PD-1 gene fragment corresponding to the-538 G/A SNP was amplified with specific primers using conventional polymerase chain reaction. immunosorbent assay. Results The frequencies of AA, GA and GG genotypes of-538 G/A SNP in LCa patients were 19.44%, 52.78% and 27.78%, respectively, compared with 31.67%, 51.67% and 16.67%, respectively, in healthy controls. Logistic regression test revealed a significant association between GG genotypes and LCa (OR= 2.85, 95%CI=1.03–7.92, P=0.045). Conclusions These results suggested the significant role of-538 G/A SNP as a risk factor for LCa in Iraqi population. Top Keywords Lung cancer, Programmed cell death protein-1, Polymorphism, Genotyping, RFLP. Top |