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Indian Journal of Public Health Research & Development
Year : 2019, Volume : 10, Issue : 7
First page : ( 496) Last page : ( 501)
Print ISSN : 0976-0245. Online ISSN : 0976-5506.
Article DOI : 10.5958/0976-5506.2019.01619.X

Diagnosis of Trichomonas Vaginalis Infection by Detection of Glutaminase (Glut) Gene by Nested PCR

Hassan Faten Ahmed1, Al-Marsomy Huda Dhaher2,*, Mustafa Suhad Asaad3

1Children Welfare Hospital, Medical City, Baghdad, Iraq

2Department of Microbiology, College of Medicine, Al-Nahrain University, Iraq

3Salahaddin University-Erbil, Kurdistan region of Iraq

*Corresponding Author: Huda Dhaher Al-Marsomy, Department of Microbiology, College of Medicine, Al-Nahrain University, Iraq, Phone: +9647703440493, Email: Hudaalmarsome@yahoo.com

Online published on 19 August, 2019.

Abstract

Trichomonas vaginalis (T. vaginalis) is a common pathogen with a cosmopolitan distribution. Trichomoniasis is associated with vaginitis, cervicitis, low birth weight, preterm delivery, and infertility. Glutaminase (glut) p3 gene which is a housekeeping gene increases the detection of T. vaginalis efficiency when used as a marker of Trichomoniasis. In this study (glut) P3 gene was depended for parasite detection by nested PCR and compared with conventional methods like wet mount and culture in the detection of T. vaginalis in vaginal discharge. Two vaginal swab specimens were obtained from 157 cases, of (mean age = 31.79 ± 9.21 years), both symptomatic and symptomatic females attending Gynecology OPD of Al-Emamayn Al-Kadhimayn Medical City in Baghdad and Maternity Teaching hospital in Erbil province, Iraq. One swab was immediately examined by wet mount microscopy and then placed in CPLM medium for cultivation, and another swab is placed in PBS buffer for PCR method. A total of 100 samples positive in one or more tests were identified: 20 (12.7%) infections were detected by wet mount microscopy, and 30 (19.1%) positives in culture. P3 nested PCR was positive in 100 (63.7%) samples. PCR appears to be the most sensitive method with high detection rate and method of choice. Furthermore nested PCR increases the sensitivity and specificity of PCR, and Glutaminase (glut) p3 gene can be efficiently used for detection of T.vaginalis.

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Keywords

T.vaginalis, Glutaminase (glut) p3 gene, nested PCR.

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