Occurrence of MBLs and Carbapenemases among MDR and XDR Acinetobacter baumannii Isolated from Hospitals in Iraq Radhi Safa Hasan1, Al-Charrakh Alaa H.1,* 1Dept. of Microbiology, College of Medicine, University of Babylon, Hilla, Iraq *Corresponding Author: Prof. Dr. Alaa H. Al-Charrakh, Department of Microbiology, College of Medicine, University of Babylon, Hilla, Iraq, Phone: 00-964-7816658072, Email: ahani67@gmail.com
Online published on 19 August, 2019. Abstract The current study was conducted to determine carbapenemase-producing Acinetobacter baumannii clinical isolates recovered from two hospitals in Iraq. A total of 540 different clinical samples were collected. Isolates were identified and Carbapenemase production were detected using MBL E-test, Modified Hodge's Test (MHT), and Modified Carba NP test. A total 30 A. baumannii isolates were obtained from clinical specimens. It was found that 73.3% isolates were carbapenem resistant. Out of 30 A.baumannii isolates, 22 (73.3%) isolates were confirmed to be MBL positive by MBL E-test method, 5(16.6%) by MHT, and 27 (90%) isolates by Modified Carba NP test. Results revealed that 21 A.baumannii isolates (70%) were found to be MDR and only 9 isolates (30%) were XDR. It can conclude that most of carbapenem resistant A.baumannii clinical isolates were MDR. The CNPt assay was the most reliable among phenotypic assays to detect MBL production. Top Keywords Acinetobacter baumannii, MBLs, Carbapenemases, MBL E-test. Top |