Effect of Exposure Biochinin-A During Gestation Stage on HoxA10 Gene Expression and Histological Change in Uterus of Healthy Female Rats Najy Huda A.1,*, Hassan Ayyed H.1 1College of Veterinary Medicine, University of Kerbala, Karbala, Iraq *Corresponding Author: Huda A. Najy, College of Veterinary Medicine, University of Kerbala, Karbala, Iraq, Email: hudaalnajy3@gmail.com
Online published on 19 August, 2019. Abstract The aim of this study is to estimate effect of exposure BC-A during gestation stage onHoxa10 mRNA expression with histological change in uterus of female rats. This study include Sixty mature female rats were meted with thirty males after making sure all female rats get pregnant. Sixty pregnant females were divided for 5 groups each groups contain 12 female rats as fallowing (control negaative, control positive, 25mg/kg, 50mg/kg, and 100mg/kg) B.W, during gestation in 12th, 14th, 16th, and 18th utero-exposure to BC A. The pregnant rats were allowed to deliver F1 pups. Adult F1femle rats were also mated with male. HoxA10 mRNA expression was measured by using RT-PCR procedure. Our result found the HoxA10 mRNA expression level were increased more in dose 100 mg/kg B.W. in uterus of female rats compared with doses 25, 50 mg/kg B.W. and control groups of F1 mature rats. While, there were histopathological changes generally including uterine hyperplasia, cystic endometrial hyperplasia (CEH), congestion and bleeding in the myometrium and enlargement in the cells lining uterine gland with reduce the number of uterine gland. In conclusion: The BC-A is exposure to during embryonic growth in uterus leads to changes in the level of gene expression of HoxA10 gene. As well as, lead to change in histological of uterus, which affects the reproductive health. Top Keywords Biochinin-A, HoxaA-10, mRNA expression, phytoestrogens, cystic endometrial hyperplasia(CEH), rats. Top |