The Antibacterial Activity of Kersen Leaves (Muntingia Calabura) Extracts against Aeromonas Hydrophilia; in Vitro Study Sari Onny Kurnia1, Tjahjaningsih Wahyu1,*, Sudarno1 1Faculty of Marine and Fisheries, Universitas Airlangga, Dharmahusada Permai No.330 street, Mulyorejo, Surabaya, East Java (60115), Indonesia *Corresponding Author: Wahyu Tjahjaningsih, Faculty of Marine and Fisheries, Universitas Airlangga, 2Dharmahusada Permai No.330 street, Mulyorejo, Surabaya, East Java (60115), Indonesia Phone: (031) 591145 Email: cahyawahyu76@yahoo.com
Online published on 19 August, 2019. Abstract Background Aeromonas hydrophila is one of opportunistic bacteria that capable causing disease in fresh water fishes. The outbreak of diseases in fresh water fishes become an important issue in fish farming. Antibiotics can be used as therapy for those problems. But the usage of antibiotics can cause the bacterial resistance and side effects of medications. The usage of antibiotics in fish farming also can trigger the water polution. Objective The aim of this study was to observe the antibacterial activity of kersen leaves Muntingia calabura extracts against A. hydrophila with in vitro study and to determine the minimum concentration the extracts that inhibit the bacterial growth and its ability to kill the bacteria. Method There was nine group consisted the extracts with various concentration, as 50%, 25%, 12, 5%, 6, 25%, 3, 12%, 1, 56%, 0, 78%, positive control group with chloramphenicol, and negative control group with dimethyl sulfoxide (DMSO) 10%. Those extract was soaked in bacterial culture and incubated for 24 hours. Result The young kersen leaves extracts had stronger antibacterial activity against A. hydrophila, compared to the old kersen leaves. Minimum Inhibition Concentration (MIC) of the extracts was at concentration of 50% and Minimum Bactericidal Concentration (MBC) was at concentration of 12, 5%. Conclusion Kersen leaves extract has antibacterial activity against A. Hydrophila by in vitro study. Top Keywords Aeromonas hydrophilia, Muntingia calabura, leaves extract, fish farming. Top |