Comparative Evaluation of Gallium Aluminium Arsenide Laser in Disinfection of Root Canal System Attavar Shruthi H1,*, Hegde Mithra N2, Shetty Veena3 1Lecturer, Department of Conservative Dentistry and Endodontics, A B Shetty Memorial Institute of Dental Science, NIITE (deemed to be University) Mangalore 2Vice Principal/And Head of The Department, Department of Conservative Dentistry and Endodontics, A B Shetty Memorial Institute of Dental Science, NIITE (deemed to be University) Mangalore 3Additional Professor, Department of Microbiology, K S Hegde Medical College, NIITE (Deemed to be University) Mangalore *Corresponding Author: Dr. Shruthi H Attavar, Lecturer, Department of Conservative Dentistry and Endodontics, A B Shetty Memorial Institute of Dental Science, NIITE (deemed to be University) Mangalore, Phone no - 8050860954, Email - drshruthiattavar@yahoo.in
Online published on 13 November, 2019. Abstract Aim To evaluate the bactericidal effect of 980 nm gallium aluminium arsenide laser and irrigating solutions such as 3% sodium hypochlorite and 2% chlorhexidine in E. faecalis infected root canals Material Method Thirtynoncarious mandibular premolar teeth were selected for the study. crowns of all the teeth were decoronated working length was determined followed by cleaning and shaping of the root canal was done using protaper size F 2. Pure bacterial culture of E faecalis (ATTCCC 29219) in BHI broth was obtained and inoculated into the root canal using insulin syringe and incubated for 48hrs.after 48 hrs root canal were disinfected using following irrigation protocol: Positive control group (n=5)-Tooth inoculated with E faecaliswith no irrigation, negative control no bacterial inoculation. Experimental group (n=10)-group I-5% sodium hypochlorite as irrigant followed by laser irradiation.group II (n=10)-2% Chlorhexidine along with laser disinfection. After disinfection, the root samples were collected using the paper point and was placed in BHI broth incubated for 24hrs. The samples were then collected from the tube and streaked into brain heart infusion agar plates using a nichrome wire loop and incubated for 24 hrs. Bacterial colony counting and scanning electron microscopic analysis was done to evaluate the efficacy of irrigation protocol on disinfection of the root canal system. Results student t-test was done to assess the level of significance between the groups. The results showed no statistically significant results between the two groups. However, chlorhexidine with laser showed less no of bacterial colonies compared to sodium hypochlorite with the laser. Conclusion within the limitation of this study both chlorhexidine with laser and sodium hypochlorite with laser can be a promising treatment option for disinfection of the root canal system. Top Keywords Laser, E faecalis, brain heart infusion broth. Top |