Detection of mecA Gene in Methicillin-Resistant Staphylococcus hemolyticus isolated from Blood cultures of Neonates in Baghdad Taha Butheina Mahamed1,*, Hussein Nadheema Hammood1, Rasool Khetam H.1, Maliki Sara Ali Jasem1, Hussein Jumaah Dakel2 1Department of Biology, College of Science, Mustansiryah University 2Medico-legal Department Ministry of Health, Baghdad, Iraq *Corresponding Author: Dr. Butheina Mahamed Taha Department of Biology, College of Science, Mustansiryah University, Email: dr.alkarkhi@gmail.com
Online published on 13 November, 2019. Abstract In this study 30 clinical isolates of Staphylococcus haemolyticus were isolated from blood cultures of neonate patients at a hospital in Baghdad and during period extended from January, 2015 to February, 2016. The of antimicrobial sensitivity test results showed the most effective antibiotics on S. haemolyticus isolates were linezolid, quinupristin and moxifloxacin which had 100% sensitivity rate, while the all isolates showed a high level of resistance to oxacillin and benzylpenicillin (100%). Phenotypic detection test of methicillin resistance to S. haemolyticus isolates showed all 30 S. haemolyticus isolates under test were methicillin resistance and the results of PCR assay for detection of mecA gene showed from 30 isolates of methicillinresistant S. haemolyticus, 28(93.3%) isolates were positive to mecA gene with 258-bp, while only 2(6.7%) isolates were negative to mecA gene. Top Keywords Staphylococcus haemolyticus, methicillin resistance and mecA Gene. Top |