Effect of Lactobacillus on the Small intestinal Mucosa Measurement of Healthy Broilers chicken Under Heat Stress condition Ali Balqees H.* College of Veterinary Medicine, University of Baghdad, Iraq *Corresponding Author: Balqees H. Ali, College of Veterinary Medicine, University of Baghdad, Iraq Email: balqees.hasan.ali@gmail.com
Online published on 13 November, 2019. Abstract Objective The present study was conducted to investigate the effect of lactobacillus bacteria on the intestinal mucosa morphology in broiler chicks under stressful environmental conditions. Materials and Method For this study, 180 broiler chicks (day-old) were randomly divided in to 2 rooms (90 chicks for each room were separated to 3 group), each group contains 30 chicks). The first room was exposed to heat stress (38–40Cº) on 3 weeks old for a period of 10 days 3 hours daily: first group (G1) received lactobacillus acidophilus 1×108cfu m1 via crop inoculation one day old; the second group (G2) received the same dose for G1 but at three weeks of age; third group (G3) was consider the control group (without any treatment). The second room also divided into 3 groups (G4, G5 and G6). This room was kept under natural condition; G4 received L. acidophilus 1×108cfu ml−1 via crop inoculation on day old; G5 received the same dose for G4 but at three weeks of age; G6 was consider the control. Collected samples at 42 days old of chicken (duodenum, jejunum and ileum). Histological techniques were applied on each sample and also the ocular micrometers were used to measure the thickness of the intestinal mucosa. Results Results showed that significantly (P<0.01) higher in the crypts of leberkuhn of duodenum in G4 compared with other groups. Chicks in G6, G2 and G1 respectively have significantly (P<0.01) higher in the crypts of lieberkuhn of jejunum. The ileum crypts of lieberkuhn was significantly (P<0.01) higher in G2 compared with other groupswhileschicks in G3, G4, G5 and G6 show significantly (P<0.01) highof the villi length of duodenum. So the villi length of jejunum show highsignificantly (P<0.01) was in G2 compared with other groups and chicks in G1 show highsignificantly (P<0.01) inthe villi length of ileum. Conclusions Taken of the lactobacillus acidophilus to the broiler chicks improving the intestinal morphology and integrity of birds subjected to the heat stress and non-heat stressed birds. Top Keywords Lactobacillus acidophilus, broiler, small intestine and heat stress. Top |