Single Nucleotide Polymorphisms in iL-10-1082 A\G Gene and Association with HcMV infection in Abortion Darweesh Hiba A. Hatem Mayyada F.* Department of Biology, Faculty of Sciences, University of Kufa, Iraq *Coresponding Author: Hiba A. Hatem Mayyada F. Darweesh, Department of Biology, Faculty of Sciences, University of Kufa, Iraq. Email: mayada.alsagheeri@uokufa.edu.iq
Online published on 13 November, 2019. Abstract Recurrent spontaneous abortion (RSA) affects approximately 1–5% of pregnancies, about third of cases were unknown cause. The production of cytokines can be influenced by genetic polymorphisms and lead to high or low levels of cytokines that subsequently play an important role in establishing a successful pregnancy. So, the present study aimed to determine whether polymorphism in IL-10-1082A/G is associated with the incidence of abortion in AL-Najaf city. Case-control study was performed to 80 patients and 40 healthy individual as control, 30 patients infected with Cytomegalovirus. Blood samples were collected from all participant. Blood used in detection IL-10-1082A\G polymorphism by amplification refractory mutation system (ARMS-PCR) Technique. IL-10 serum level were measured by enzyme-linked immunosorbent assay test(ELISA). The result explain that the age group (20–29) and at three time of recurrent abortion was more infected with CMV. SNP in IL-10-1082A/G were AA (60%), AG (27.5%)) and GG (12.5%) in patients as compared to control (15%), (30%) and (55%) respectively. AA genotype associated with low serum level of IL10 (2.5 pg/ml) while GA and GG genotype associated with high serum level (5pg/ml and 12. 3 pg/ml) respectively. This result shown that IL-10 level is significantly decrease in aborted patients than control, and AA genotype is risk factor for abortion. Conclusion The polymorphism in IL-10 at position (1082A\G) has association with abortion at AA genotype, GG consider as protective factor. decrease IL-10 levels in aborted women if CMV infected or non-infected. Top Keywords IL-10, polymorphism, ARMS, abortion, HCMV. Top |