Effect of Dexamethasone on Marbofloxacin Residues in Rabbit Tissues Using HPLC Shams Gamal A.1, Ibrahim Hosny A.1, Darwish Ashraf S.2, Mahmoud Hend A.3,* 1Pharmacology Department Faculty of Veterinary Medicine, Zagazig University, Egypt 2Biochemistry, Toxicology and Feed Deficiency Department Pharmacology Unit, Animal Health Research Institute, Dokki, Giza, Egypt 3Biochemistry, Toxicology and Feed Deficiency Department Pharmacology Unit, Animal Health Research Institute, Zagazig Branch *Corresponding Author Hend A. Mahmoud Biochemistry, Toxicology and Feed Deficiency Department, Pharmacology Unit, Animal Health Research Institute, Zagazig Branch e-mail: hendahmed0055@yahoo.com
Online published on 13 November, 2019. Abstract The present study sought to investigate the residues of marbofloxacin in different tissues of healthy rabbits as well as to detect the effect of dexamethasone on tissue residues of marbofloxacin when co-administrated together after multiple intramuscular administrations. Forty-five rabbits were divided into three equal groups (n=15); the first group, healthy male New Zealand rabbits were untreated and served as control, the second group was injected with marbofloxacin intramuscular at a dose of 2mg/kg b.wt for 5 consecutive days in the thigh muscle, and the third group was co-administrated marbofloxacin and dexamethasone intramuscularly with the same dose and same period. Samples were collected on the 1st, 3rd, 5th, 7th and 9th day from rabbit tissues (Liver, kidneys, breast muscle, thigh muscles, and lung). Samples from each rabbit were extracted and marbofloxacin residues were analyzed using HPLC with ultraviolet detection. The obtained result emphasized a widespread distribution of marbofloxacin in the tested tissues. The highest concentration (0.402+0.007 μg/gm), (0.406± 0.010 μg/gm) of marbofloxacin alone or co-administered with dexamethasone was measured in kidney tissue respectively, followed by liver (0.324±0.016 μg/gm), (0.313± 0.019 μg/gm), lung (0.204±0.01 μg/gm), (0.205±0.05), breast muscle (0.079±0.003), (0.080±0.006) and thigh muscle (0.0635±0.002), (0.0655±0.007) respectively with no significant changes between two groups. Marbofloxacin remained within detectable limit till the 3rd day in most tested tissues, while in kidneys and liver, it remained till the 5thday following the last intramuscular dose. The obtained results provide a basis for the rational use of dexamethasone with marbofloxacin in rabbit medicine to treat the sensitive bacterial infection with inflammatory conditions. Top Keywords Marbofloxacin, Dexamethasone, Residues, HPLC, Rabbits. Top |