Isolation and Identification of Streptococcus mutans from Dental Caries by Using SM479 gene Al-Mohammadawy Zainab H1, Aljarah Ali Kadhim2, Saad Ali Malik1 1Department of Biology, College of Science for Women, University of Babylon, Iraq 2Branch of Microbiology, College of Veterinary Medicine, University of Kerbala, Iraq Online published on 1 November, 2018. Abstract About 165 samples collected from patients with dental caries infections from the Specialty Center of dentist in Hilla city during period 1/10/2017 to 10/2/2018, only 120 sample give positive result on M S B A media, Streptococcus was noticed with high ratio, Streptococcus mutans (51%), Streptococcus mitis (28%) and Streptococcus salivarius (21%). All specimens were cultured on MSA agar with Bacitracin for isolation and detection of S. Mutans, then altered by the isolation on sub cultured (blood agar)for the detection of the hemolysis type to recognized Streptococcus mutans colony. For more detection of isolates antibiotic sensitivity test has been completed to show the resistance for antibiotic activity such as (Optechin, Amoxicillin and Bacitracin). Genetic detection was done by using specific gene for Streptococcus mutans this gene was the (sm479 gene) between all genus of Streptococci. Top Keywords Dental caries, Streptococcus mutans, Genetic detection, PCR amplification, Sm479 gene. Top |