Identification of Active Pharmaceutical Ingredients in Thevetia neriifolia Juss Leaf Callus using Analysis of GC-MS Salim Siham A. Al-Musaib Technical College, Al-Furat Al-Awsat Technical University, Iraq Online published on 2 February, 2019. Abstract Plants of Thevetia neriifolia are recorded from the most important medicinal plants that used in folklore medicine in different countries. Most of its parts were used to treat different diseases and having antioxidant, antimicrobial and anti-infammations activities. Callus induction was occurred by culturing of leaf explants that were taken from eight years old trees on Murashige and Skoog(MS) medium supplemented with the auxin 2, 4-D at 3.0 mg. l−l. After the gaining of suitable amount of callus and extracted with hexane, the screening of active phytochemica1s was achieved by using the analysis of GC-MS. The results showed the presence of forty different compounds that diagnosed in the callus extract with different retention times and peak areas, the main compounds that having various pharmaceutical features including: Hexatriacontane (23.095%), Nonacosane(8.849%),.beta-Amyrin(8.060%), 2-Methylhexacosane(6.870%),.alpha.-Amyrin(4.446%), Octacosane (4.309%), Phenol, 2, 2′-methylenebis-(l, l-dimethylethyl)-4-methyl-(4.030%), Vitamin E (3.134%),.beta.-Sitosterol(3.254%), Isopropyl myristate(2.777%), 1, 2-Benzisothiazol-3-amine tbdms (2.886%), Pentatriacontane(1.716%) Squalene(1.170%). Top Keywords Phytochemicals, medicinal plants, GC-MS technique, Thevetia neriifolia, callus. Top |