Resistance Status in Aedes Aegypti Strain from North Toraja, Indonesia to Malathion and Temephos Insecticides Ishak Hasanuddin1, Ponno Sepri2 1Department of Environmental Health, Hasanuddin University, Makassar, Indonesia 2Graduate School of Public Health, Faculty of Public Health Hasanuddin University, Makassar, Indonesia Online published on 2 February, 2019. Abstract Organophosphates is the main choice in controlling Aedes aegypti mosquito as the main vector of Dengue Hemorrhagic Fever. This study aims to determine resistance status of Aedes aegypti collected North Toraja District to malathion and temephos. This type of research is a quasi experiment. Assays to determine resistance status were conducted using WHO standard method. The samples were 600 Aedes aegypti F1 3rd and 4th instar larvae; and adult Aedes aegypti F1 female mosquitoes aged 3–7 days obtained from ovitrap survey at high dengue endemic area (Rantepao sub-district) and non endemic area (Sanggalangi sub-district) in North Toraja District. The adult samples were tested to Malathion 0.8% and 5%. The larva samples were tested to Temephos 1% concentration with 3 times replication, the number of each test was 20 mosquitoes. The statistical test used is univariate and probit analysis. The results showed that Aedes aegypti (high endemic strain) adults was resistance to Malathion 0.8% concentration, and that was tolerance to Malathion 5% concentration, whereas Aedes aegypti larvae was susceptible to Temephos 1%. Aedes aegypti (non endemic strain) were susceptible to Malathion 0.8%, Malathion 5% and Temephos 1%. There were a difference in lethal time (LT50, LT90, LT95, and LT99) between Aedes aegypti high endemic and none endemic strains to both malathion and temephos. The value of lethal time of Aedes aegypti high endemic strain were higher than those non endemic strain Top Keywords Resistance test, malathion, temephos, Aedes aegypti. Top |