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Indian Journal of Public Health Research & Development
Year : 2018, Volume : 9, Issue : 12
First page : ( 1810) Last page : ( 1815)
Print ISSN : 0976-0245. Online ISSN : 0976-5506.
Article DOI : 10.5958/0976-5506.2018.02252.0

Phenanthrene Biodegradation Efficiency of Bacillus sp. P4a Isolated from Hydrocarbon Contaminated Soil

Fazila A.1, Ismail N.2, Darah I.3

1School of Fundamental Science, Universiti Malaysia Terengganu, Malaysia

2Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Malaysia

3Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia

Online published on 2 February, 2019.

Abstract

In this study, phenanthrene-degrading bacteria was successfully isolated from hydrocarbon contaminated soil. The bacteria were identifed based on their morphological, biochemical activities and molecular identifcation using 16S rRNA. The phenanthrene-degrading capabilities was cultivated in an enrichment culture containing phenanthrene as a sole source of carbon and energy. The phenanthrene degradation was analysed using Gas Chromatography-Flame Ionization Detector (GC-FID) and the result obtained indicated that the Bacillus sp. P4a was capable of degradingto about 83.01% in the enrichment culture. The Bacillus sp. P4a has been shown to degrade phenanthrene via salicylate pathway which produced salicylic acid and catechol compound after14 and 28 days of incubation. The result revealed that this bacterial isolate can be highly recommended for bioremediation of hydrocarbon contaminants due to their capability to degrade phenanthrene.

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Keywords

Phenanthrene, Bacillus sp. P4a, Biodegradation, Bioremediation, Contaminated soil.

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