Anti-Candidal Activity of 5 Rare Plants against Drug Resistance Candida Species Sahu Mahesh Chandra1,*, Sahoo Swayamprabha2 1Assistant Professor Medical Research Laboratory IMS and SUM hospital, Siksha “O” Anusandhan Deemed to be University, K8, Kalinganagar, Bhubaneswar, Odisha, India 2Research scholar, Medical Research Laboratory IMS and SUM hospital, Siksha “O” Anusandhan Deemed to be University, K8, Kalinganagar, Bhubaneswar, Odisha, India *Corresponding Author: Dr. Mahesh Chandra Sahu, PhD, NPDF, Assistant Professor, Medical Research Laboratory, IMS and SUM hospital, Bhubaneswar. Email: mchsahu@gmail.com
Online published on 2 February, 2019. Abstract Candida species is the fourth common cause of blood stream infections all over the world which is life threatening. Invasive candidiasis leads to increased mortality and morbidity especially in immunosuppressed. The antifungal resistance pattern in high-risk patients is major concern.. The present study was to access the anticandidal activity of leaves of 5 medicinal plants against fluconazole resistant Candida species and C. albicans isolated from patients of IMS and SUM Hospital. Ethanol, chloroform, petroleum ether and aqueous extracts of leaves, bark and seeds of C. fistula linn. was evaluated against Microbial type culture collection (MTCC) Candida strains and 21 fluconazole resistant clinical isolates. Antifungal activity was evaluated by agar diffusion and broth dilution techniques. The active phytochemical component present in the ethanol extract of seeds was accessed by high performance thin layer chromatography. The docking study was done with lanosterol 14-alpha demethylase, the azole drug target with the predominant phytochemical from the extract having antifungal activity. Top Keywords Antifungal activity, candidiasis, medicinal plants, MIC, MFC, resistant Candida strains. Top |