Surveillance of Ciprofloxacin Resistance Bacteria in Chronic Suppurative Otitis Media Rath Pritam Priyadarshi1, Sahoo Swaymprabha1, sahu Mahesh Chandra2,* 1Research Scholar, Medical Research Laboratory IMS and SUM Hospital, Siksha ‘O’ Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India 2Assistant Professor, Medical Research Laboratory IMS and SUM Hospital, Siksha ‘O’ Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India *Corresponding Author: Dr. Mahesh Chandra Sahu, PhD, NPDF, Assistant Professor, Medical Research Laboratory, IMS and SUM hospital, Bhubaneswar, Email: mchsahu@gmail.com
Online published on 2 February, 2019. Abstract Background To isolate causative bacteria and antibiotic sensitivity pattern for CSOM and confirmed with 16S rRNA for identification of bacteria. The ciprofloxacin resistant bacteria were identifies with molecular markers. Materials and Method A total of 115 ear swabs of clinical suspected CSOM patients were cultured on specific cultured medium and identified the bacteria with both conventional and 16S rRNA methods. Then all the identified bacteria were subjected with specific antibiotics by the Kirby-Bauer's method to know the resistance pattern of antibiotics. With specific primers (molecular marker), we have identified the ciprofloxacin resistance bacteria. Results A total of 103 bacteria were isolated from 112 CSOM patients among them P. aeruginosa (29.17%), Staphylococcus aureus (22.40%), A. baumannii(13.80), E. aerogenes(18%), C. freundii (6.77%), K. oxytoca (6.25%), P. vulgaris (5.99%), K. pneumoniae (4.69%) and P. mirabilis (4%) were identified. From antibiotic disc diffusion methods 79.46% ESBL strains and 9.90% MBL strains were documented. Multidrug resistant strains of P. aeruginosa were more prevalent than those of S. aureus and other bacteria in ear discharges. The commonly used antibiotic ciprofloxacin was 55% resistant. The gene responsible for ciprofloxacin resistant gene were identified with synthesized molecular markers. Conclusion Continuous and periodic evaluation of microbiological profile and antimicrobial sensitivity pattern of bacterial is essential for optimum management of CSOM patients. Empirical use of antibiotic should not be practiced while prescribing drugs. PCR method should be implemented for early detection of ciprofloxacin resistance bacteria. Top Keywords Antibiotics, CSOM, MDR, Gram negative bacteria, Drug resistance. Top |