Histopathological alterations associated with immunolocalization of Clostridium perfringens and Salmonella spp in neonatal bovine calves died of diarrhoea Raksha Suresh, Brar A.P.S., Sood N.K., Leishangthem Geeta Devi*, Jaiswal Vikas Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India *Address for Correspondence, Dr Geeta Devi Leishangthem, Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141012, Punjab, India, E-mail: drgeetapatho@gmail.com
Online Published on 28 July, 2022. Abstract Clostridium perfringens and Salmonella spp are the major bacterial enteropathogens responsible for causing diarrhoea in calves. The aim of the study was to detect Clostridium perfringens and Salmonella spp by immunohistochemical technique (IHC) and to study the associated histopathological changes in jejunum and ileum of calves that died of neonatal calf scours. Ninety samples of intestine from the diarrhoeic neonatal calves were collected and processed for routine histopathology and immunohistochemistry. Out of these 90 samples examined, 21 samples were positive for Clostridium perfringens, 22 were positive for Salmonella spp and 25 were found to be positive for both the organisms by immunohistochemistry. Histopathological alterations such as altered villi: crypt ratio, desquamation of epithelium, congestion, intactness of muscularis mucosae, crypt hyperplasia, crypt destruction, fibrosis and severity of inflammation were observed and scoring was done in relation to Salmonella spp and C. perfringens. The total histopathological score in samples of mixed infections was higher as compared to the total score of samples with single infection indicating co-infections of Salmonella spp and C. perfringens can lead to severe enteritis in calves. The study was highly useful in immunolocalization of C. perfringens and Salmonella spp and to ascrible the pathological lesions to the etiology. Top Keywords Clostridium perfringens, Histopathology, Immunohistochemistry, Neonatal calf diarrhoea, Salmonella spp. Top |