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INTAS POLIVET
Year : 2018, Volume : 19, Issue : 1
First page : ( 61) Last page : ( 63)
Print ISSN : 0972-1738. Online ISSN : 2249-8796.

Evaluating peritoneal fluid microbial profile in cattle with intestinal obstruction-pre and post surgery

Kumar Ashok1,*, Bhardwaj H.R.2, Dwivedi D.K.1, Sharma H.K.1

Division of Veterinary Surgery and Radiology Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agriculture Science and Technology (SKUAST-J), R.S. Pura, Jammu-181102 (Jammu and Kashmir)

1Assistant Professor Division of Veterinary Surgery and Radiology Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agriculture Science and Technology (SKUAST-J), R.S. Pura, Jammu-181102 (Jammu and Kashmir)

2Associate Professor Division of Veterinary Surgery and Radiology Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agriculture Science and Technology (SKUAST-J), R.S. Pura, Jammu-181102 (Jammu and Kashmir)

*Corresponding author. E-mail: ashokskuast@gmail.com

Online published on 5 November, 2018.

Abstract

The study was conducted on ten cattle presented for treatment of digestive tract disorders. These animals were diagnosed suffering from intestinal obstruction on basis of history, clinical signs and trans-rectal examination. Further confirmation of diagnosis was made by subjecting these animals to right flank laparotomy. The intussuscepted mass was palpated and exteriorized. The peritoneal fluid was collected at time of right flank laparotomy, twenty four hours and forty eight hours after surgical operation. The peritoneal fluid samples collected were analysed for isolation and identification of bacterial biomass, both quantitatively and qualitatively. The number of bacterial biomass isolated from peritoneal fluid at time of surgery, 24 and 48 hours after surgery was 104–4.25, 104–4.5 and103–3.5 colony forming unit (CFU)/ml of peritoneal fluid. Peritoneal fluid of cattle suffering from intestinal obstruction at time of surgery comprised of colonies of Staphylococcus aureus and Streptococcus spp. The bacterial biomass isolated from peritoneal fluid of cattle suffering from intestinal obstruction twenty four hours after surgery comprised of colonies of Staphylococcus aureus and Bacillus cereus. The bacterial biomass isolated from peritoneal fluid of cattle suffering from intestinal obstruction forty eight hours after surgery comprised of colonies of Staphylococcus aureus and Bacillus cereus.

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Keywords

Cattle, intestinal obstruction, microbial profile, peritoneal fluid.

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