Isolation and Molecular Characterization of BCSP-31 Gene of Brucella abortus from Bovine Abortions Singh Chhabil1,*, Sharma Rahul2, Sagi S.B. RamaRaju2, Thakur Vikas3, Kumar Amit4 Central Military Veterinary Laboratory (CMVL), Sardhana Road Meerut Cantt. Meerut-250001 (Uttar Pradesh) 1Lab Officer, RVS (Tech), RV Branch, IHQ of MoD 2Lab Officer, RVS (Tech), RV Branch, IHQ of MoD 3Commandant, RVS (Tech), RV Branch, IHQ of MoD 4Dir, RVS (Tech), RV Branch, IHQ of MoD *Corresponding author. E-mail: drchhabilsingh@gmail.com
Online published on 4 May, 2019. Abstract The study reports isolation, identification and molecular detection of Brucella abortus. Four (04) clinical samples positive for Brucella abortus by culture isolation were further confirmed by PCR. Molecular characterization of Brucella abortus BCSP-31 gene (protein which frequently elicits an immune response during infection) was successfully done. Top Keywords BCSP-31, Brucella abortus, isolation, PCR, sequencing. Top |