Biogenic synthesis of silver nanoparticles with Grewia orbiculata (GO@AgNPs) and evaluation of their bioactive potential Suguna M.**, Umesha S.* Department of Biotechnology, University of Mysore, Manasagangotri, Mysore-570006, Karnataka, India *Corresponding author e-mail: pmumesh@gmail.com
**su@appbot.uni_mysore.ac.in
Online Published on 31 March, 2023. Abstract Silver nanoparticles (GO@AgNPs) were biogenically produced using Methanolic Extract of Bark (MEB) of the plant Grewia orbiculata. Initially GO@AgNPs were synthesised and characterised using UV-spectrometer. The physicochemical stability was determined to be robust and unaltered in terms of pH, temperature, and salinity changes. GO@AgNPs were also evaluated for their biological potential. The nanoparticles displayed strong radical scavenging capacities in the 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2’-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS) assays, with IC50 values of 4.5 µg/mL and 3.5 µg/mL, respectively. The Ferric Reducing Antioxidant Power Assay (FRAP) assay revealed that it has outstanding metal chelating activity, with a value of 6 µg/mL. The GO@AgNPs were found to have excellent and enhanced antimicrobial potency than the crude extract against 10 human pathogens tested, namely Staphylococcus aureus, Methicillin resistant Staphylococcus aureus, Streptococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, and Mycobacterium smegmatis. It was also reported to demonstrate remarkable anti-cancerous property against A549 lung carcinoma cells at a low dosage of 46 µg/mL, while being non-toxic to normal cell lines such as tracheal epithelial cells even at highest conc. of 320 µg/mL tested to assess the cytotoxicity. They were proven to be biocompatible with plasma, as GO@AgNPs exhibited no substantial agglutination of RBCs up to a concentration of 50 mg/mL, and in vitro anti-inflammatory effect of GO@AgNPs revealed protection against BSA denaturation up to a concentration of 100 mg/mL. These results indicate that GO@AgNPs might be employed in biological and pharmacological applications. Top Keywords Antioxidant property, Anti-cancerous activity, Antibacterial activity, In vitro anti-inflammatory activity, Cytotoxicity. Top |