Relationship between the Dynamics of Oxidative Stress and Thyroid State Kale MK1,*, Bhusari KP1, Umathe SN2 1Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur 2Department of Pharmaceutical Sciences, R.T.M. Nagpur University, Amaravati Road, Nagpur *Corresponding Author E-mail: kalemkncp@rediffmail.com
Abstract To examine the possible relationship between the dynamics of oxidative stress and thyroid state to evaluate role of thyroxine in oxidative stress. The Sprague-Dawley rats of either sex were equally divided in to three groups: control, hypothyroid, and hyperthyroid. Hypothyroidism was induced by administering methimazole in drinking water (0·02% w/v) for 15 days. Hyperthyroidism was induced by daily i.p. administration of tri-iodothyronine (T3) (100 μg/kg body weight) for 10 days in methimazole treated rats to induce mild hyperthyroidism in hypothyroid rats. At the end of the protocol, blood was withdrawn from retro-orbital plexus. The parameters like lipid peroxidation, superoxide dismutase and catalase were determined in erythrocyte lysate while reduced glutathione was determined in blood to find out the extent of oxidative stress. Same blood was utilized for testing of thyroid function by determining the levels of T3, T4 & TSH. We found suppressed TSH level, with elevation of T4 and T3, increased lipid peroxidation and decreased superoxide dismutase, catalase and glutathione in hyperthyroid, as observed in oxidative stress. These were reversed with methimazole, an antithyroid in hypothyroid rats. Hence, there exist some relationship between the dynamics of oxidative stress and thyroid state. Reports as well as our findings suggest that hyperthyroidism increases oxidative stress. Treatment with thyroxin produces oxidative stress, which is reversed in hypothyroidism. Hence, it is contemplated that there exist some relationship between the dynamics of oxidative stress and thyroid state Top Keywords Free Radicals, Oxidative Stress, Antithyroid, and Thyroid gland. Top |