Identification and Quantification of six natural compounds from Picrorhiza kurroa leaf extract and their Antibacterial and Antioxidant activity Singh Gajender1, Bisht Dheeraj2, Arya Rajeshwar Kamal Kant2, Kumar Sunil1, Kumar Deepak1,**, Dash Ashutosh K.3,* 1Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan - 173229, India. 2Department of Pharmaceutical Sciences, Sir J. C. Bose Technical Campus Bhimtal, Kumaun University, Nainital - 263136, Uttarakhand, India. 3Devsynthesis India Pvt. Ltd, Drug Discovery Chemistry CRO, Hyderabad, India – 500076. *Corresponding Author E-mail: ashutosh.dash10@gmail.com
**guptadeepak002@gmail.com
Online Published on 06 March, 2023. Abstract Picrorhiza kurroa (kutki) is a hairy herb belonging to Plantaginaceae/ Scropulariacea family and found in some parts of India (Kashmir to Sikkim), Nepal, and China.They found application in various ailments such as inflammation, antioxidant activity, asthmatic activity, hepatic activity, etc. The compound constituents using HPLC retrieved the presence of eighteen compounds. HPTLC analysis confirmed and quantified the presence of six major compounds. The quantity of diosgenin was found to be (2.80%) maximum followed by chlorogenic acid (2.70% w/w of extract) in the DCM/Methanol leaf extract. Antibacterial activity was performed using the zone of inhibition method for eight different leaf extracts of Picrorhiza kurroa. Among the extracts, ethyl acetate extract showed good potency in terms of zone of inhibition (13.33±1.52) against S. aureus whereas n-butanol extract showed good potency (12.01±1.00) against S. mutans, acetone extracts showed good potency (16.66±.47) against E. coli, acetone extracts showed good potency (11.33±1.52) against K. pneumoniae and acetone extracts showed good potency (11.00±1.00) against P. aeruginosa. Methanol extract showed good antioxidant activity (41.3±3.1µg/ml) whereas n-hexane showed the least (389.5±1.3µg/ml). Chlormphenicol and Ascorbic acid were taken as standard in the antibacterial and antioxidant study. IC50 values were calculated from the known protocol. Top Keywords Picrorhiza kurroa, Antibacterialactivity, Antioxidant activity, Chloramphenicol, Ascorbic acid. Top |