Phytochemical, Antioxidant and Antidiabetic properties of Senna alexandrina Leaf Extract Rosmalena Rosmalena1,*, Senlia Aldo O.1, Hanafi Muhammad2,3, Artanti Nina3, Eldafira Eldafira4, Handayani Supri I.5, Lotulung Puspa D.5, Hartati Sri5, Elya Berna6, Zulfa Astri7, Prasasty Vivitri D.8,** 1Department of Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta10440, Indonesia. 2Research Centre for Chemistry – BRIN, Indonesian Institute of Sciences, South Tangerang15314, Indonesia. 3Faculty of Pharmacy, Pancasila University, Jakarta12640, Indonesia. 4Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta10440, Indonesia. 5Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta10440, Indonesia. 6Faculty of Pharmacy, Universitas Indonesia, Depok, West Java16424, Indonesia. 7Faculty of Biology, Universitas Nasional, Jakarta, Indonesia12520, Indonesia. 8School of Basic Pharmaceuticals and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, Louisiana71201, United States. *Corresponding Author E-mail: rosmalena2018@gmail.com
**prasasty@ulm.edu
Online Published on 06 March, 2023. Abstract Diabetes is a growing problem in modern times. Treating diabetes can be done using various ways, from traditional to modern methods. Herbal medicine is a traditional form of medication. Herbs can be used to treat diabetes, such as Senna alexandrina. The effects of herbal medicine are linked with the solvent used. In making a herbal drink on an industrial and private scale, the solvent used is ethanol and water. This study aimed to determine the best solvent to produce antioxidant and antidiabetic effects from S. alexandrina leaves. In vitro analysis using DPPH and α-glucosidase enzyme was done to find the antioxidant and antidiabetic properties. S. alexandrina leaves were macerated using ethanol and water. All extract was screened for phytochemical contents, and the bioactive compounds were profiled using LC-MS/MS. Total phenolic and flavonoid contents were determined using gallic acid and quercetin as standard compounds. S. alexandrina leaves showed antioxidant and antidiabetic properties. Ethanol 50% extract showed the best antioxidant and antidiabetic properties, followed by ethanol and water extracts. The IC50 value of the antidiabetic activity of ethanol 50% was 33.151 µg/mL and the IC50 value of antioxidant power was 160.502 µg/mL. The bioactive compounds were profiled in this extract including torachrysone-8-O-β-D-glucopyranoside, oroxin B, 3-O-[β-D-glucopyra-nosyl-(12)]-β-D-glucopyranosyl-kaempferol, 7-hydroxy-1-methoxy-2-methoxyxanthone, rhamnetin dan rubilactone. Top Keywords Senna alexandrina, Antioxidant, α-glucosidase, In vitro, Antidiabetes. Top |