Isolation and identification of antibacterial compound from actinomycetes isolated from mangrove soil Jalan Riya1, Pradhan Biswaranjan2, Singh Santosh Kumar3, Das Anuradha4, Barik Rajib Lochan5, Meher Jyotisikha1, Mishra Rashmi Ranjan1, Dubey Debasmita6, Behera Bikash Chandra1,* 1Department of Biotechnology, MITS School of Biotechnology, Orissa - 751024, India 2S.K. Dash Centre of Excellence of Biosciences and Engineering and Technology, Indian Institute of Technology, Bhubaneswar - 752050, India 3Department of Biotechnology, School of Health and Allied Sciences, ARKA JAIN University, Jamshedpur - 831001, India 4School of Chemical Sciences, National Institute of Science Education and Research, Bhubaneswar - 752050, India 5District Public Health LaboratoryDistrict Head Quarters Hospital, Baripada - 757001, India 6Department of Biotechnology and Bioinformatics, Sambalpur UniversityJyotivihar - 768019, India *Corresponding Author E-mail: bikashnou@gmail.com
Online Published on 08 June, 2022. Abstract Nine actinobacteria were isolated from the mangrove soil of Bhitarkanika and screened for their bioactive metabolites and antimicrobial property. Out of the nine actinobacterial isolates, the bacterial isolateBAB-7 showed positive activity towards most of the enzymesscreened,i.e., Cellulase, phosphate solubilization, L-asparaginase, protease, and amylase. The actinobacterial isolate BAB-7 also exhibited antimicrobial activity against K. pneumoniae, V. alginolyticus, V. parahaemolyticus, P. aeruginosa,E. coli, S. typhimurium, and S. salivarius. Based on biochemical characterization, the actinobacterial isolate BAB-7 was identified as Steptomcessp. The secondary metabolites produced by the bacterial strain were analyzed through GC-mass spectroscopy and found to besimilar to the peaks of2-cyclohex-3-en-1-yl-2-oxoacetic acid derivative (ketomycin). Top Keywords Antibiotics, Bioactive metabolites, Actinobacteria, Mangrove. Top |