Comparison of diagnostic tests for detection of Nonstructural-1(NS1) antigen dengue virus using immunochromatography and fluorescence immunoassay methods Zuroidah Nelly1, Tanzilia May Fanny2, Sunari I Gusti Agung Ayu Eka Putri1, Wrahatnala Billy Jordan3, Haki Faradila Khoirun Nisa3, Aryati4,5,*, Wardhani Puspa4,5, Husada Dominicus6, Rohman Ali7, Tarmizi Siti Nadia8 1Clinical Pathology Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 2Clinical Pathology Sub-Specialization Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 3Master Program of Tropical Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 4Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 5Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia 6Department of Pediatric, Faculty of Medicine - Universitas Airlangga, Surabaya 7Department of Chemistry, Faculty of Science and Technology - Universitas Airlangga, Surabaya 8Directorate of Vector Borne and Zoonotic Disease, Ministry of Health Republic of Indonesia, Jakarta, Indonesia *Corresponding Author E-mail: aryati_dr@yahoo.com
Online Published on 08 June, 2022. Abstract Background NS1 is currently widely used for diagnosis of dengue virus (DENV) infection. Various methods are used to diagnose DENV infection (DVI), either ELISA, immunochromatography (ICT) or most recently the fluorescence immunoassay (FIA) method which are commercially available. Objective This study aimed to compare the detection capabilities of dengue NS1 antigens using (1) Dengue NS1 ICT Ag (Standard Q - SD Biosensor, Inc.), (2) Dengue NS1 ICT Ag (SD Bioline - Standard Diagnostic, Inc), and (3) Dengue NS1 Ag FIA (Standard F - SD Biosensor, Inc.) Methods This study consisted of serum samples (n=80) with the number of DVI patients (n=50), non-DVI (n=30). All samples were examined using all three commercial kits for NS1 antigen testing. All DVI samples showed results of reverse-transcriptase polymerase chain reaction (RT-PCR -SIMPLEXA™ Dengue - Focus Diagnostics) and/or positive dengue NS1 (Panbio® Dengue Early ELISA) antigen. Results: Standard F showed the highest sensitivity (82%) compared to Standard Q (74%) and SD Bio line (74%). These three commercial kits had the same specificity 100%. The positive predictive value all of these kits was 100% each. The negative prediction value of Standard F, Standard Q, and SD Bio line were 76.9%, 63.8%, 63.8%, respectively. These three NS1 antigen tests had a good agreement (k 0.681–0.774). Conclusions FIA test performance (Standard F SD - Biosensor, Inc.) were a quick and easy examination, showing a higher sensitivity and specificity than ICT for detecting DENV infection. Further research is needed to confirm the diagnosis of primary or secondary infection. Top Keywords Dengue Viral Infection, NS1 Dengue antigen, ICT, FIA. Top |